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      <title>Biomedical and Environmental Sciences</title>
    <link>/</link>
    <description><![CDATA[《Biomedical and Environmental Sciences》2024年第6期]]></description>
    <year><![CDATA[2024]]></year>
    <volume><![CDATA[37]]></volume>
    <issue><![CDATA[6]]></issue>
    	    <item>
	       	<title>2024-6 Cover</title>
	      	<link>//article/id/adfc2314-acff-465e-b9fe-c5362fafdbee</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage></startPage>
	      	<endPage></endPage>
	      	<author>
				
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    	    <item>
	       	<title>2024-6 Contents</title>
	      	<link>//article/id/fca3c5cb-23ff-4711-9af3-7a073e343a3b</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>1</startPage>
	      	<endPage>2</endPage>
	      	<author>
				
	      	</author>
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    	    <item>
	       	<title>Message from the New Editor-in-Chief</title>
	      	<link>//article/id/be3b6969-ea5c-450e-85bf-5bc8eb34f493</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>559</startPage>
	      	<endPage>562</endPage>
	      	<author>
				Xiaoming Shi
	      	</author>
	    </item>
    	    <item>
	       	<title>Uphold the Original Intention and Work together for Future Development — Speech at the 35&amp;lt;sup&amp;lt;th&amp;lt;/sup&amp;lt; Anniversary Symposium of &amp;lt;i&amp;lt;BES&amp;lt;/i&amp;lt;</title>
	      	<link>//article/id/2f3046d4-8d00-4fac-b6c0-1bebe714c246</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>563</startPage>
	      	<endPage>564</endPage>
	      	<author>
				Dr. Junshi Chen
	      	</author>
	    </item>
    	    <item>
	       	<title>Identifying Comprehensive Genomic Alterations and Potential Neoantigens for Cervical Cancer Immunotherapy in a Cohort of Chinese Squamous Cell Carcinoma of the Cervix</title>
	      	<link>//article/id/db59f7d3-798b-4ebb-a68e-52b0b94ea8c1</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp; Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma (CSCC). &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp; Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC immunotherapy. RNA Sequencing was performed to analyze neoantigen expression. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp; Systematic bioinformatics analysis showed that C&amp;gt;T/G&amp;gt;A transitions/transversions were dominant in CSCCs. Missense mutations were the most frequent types of somatic mutation in the coding sequence regions. Mutational signature analysis detected signature 2, signature 6, and signature 7 in CSCC samples. &amp;lt;i&amp;lt;PIK3CA&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;FBXW7&amp;lt;/i&amp;lt;, and &amp;lt;i&amp;lt;BICRA&amp;lt;/i&amp;lt; were identified as potential driver genes, with &amp;lt;i&amp;lt;BICRA&amp;lt;/i&amp;lt; as a newly reported gene. Genomic variation profiling identified 4,960 potential neoantigens, of which 114 were listed in two neoantigen-related databases.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp; The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>565</startPage>
	      	<endPage>580</endPage>
	      	<author>
				Meng Wu, Jialu Zhou, Zhe Zhang, Yuanguang Meng
	      	</author>
	    </item>
    	    <item>
	       	<title>Predicting the Prognosis and Immunotherapeutic Response of Triple-Negative Breast Cancer by Constructing a Prognostic Model Based on CD8+ T Cell-Related Immune Genes</title>
	      	<link>//article/id/85cb2456-bd80-43e5-9bc8-f672e4cc1a05</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;Triple-negative breast cancer (TNBC) poses a significant challenge for treatment efficacy. CD8+ T cells, which are pivotal immune cells, can be effectively analyzed for differential gene expression across diverse cell populations owing to rapid advancements in sequencing technology. By leveraging these genes, our objective was to develop a prognostic model that accurately predicts the prognosis of patients with TNBC and their responsiveness to immunotherapy.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Sample information and clinical data of TNBC were sourced from The Cancer Genome Atlas and METABRIC databases. In the initial stage, we identified 67 differentially expressed genes associated with immune response in CD8+ T cells. Subsequently, we narrowed our focus to three key genes, namely &amp;lt;i&amp;lt;CXCL13&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;GBP2&amp;lt;/i&amp;lt;, and &amp;lt;i&amp;lt;GZMB&amp;lt;/i&amp;lt;, which were used to construct a prognostic model. The accuracy of the model was assessed using the validation set data and receiver operating characteristic (ROC) curves. Furthermore, we employed various methods, including Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, immune infiltration, and correlation analyses with CD274 (PD-L1) to explore the model&apos;s predictive efficacy in immunotherapeutic responses. Additionally, we investigated the potential underlying biological pathways that contribute to divergent treatment responses.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;We successfully developed a model capable of predicting the prognosis of patients with TNBC. The areas under the curve (AUC) values for the 1-, 3-, and 5-year survival predictions were 0.618, 0.652, and 0.826, respectively. Employing this risk model, we stratified the samples into high- and low-risk groups. Through KEGG enrichment analysis, we observed that the high-risk group predominantly exhibited enrichment in metabolism-related pathways such as drug and chlorophyll metabolism, whereas the low-risk group demonstrated significant enrichment in cytokine pathways. Furthermore, immune landscape analysis revealed noteworthy variations between (PD-L1) expression and risk scores, indicating that our model effectively predicted the response of patients to immune-based treatments.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;Our study demonstrates the potential of &amp;lt;i&amp;lt;CXCL13, GBP2,&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;GZMB&amp;lt;/i&amp;lt; as prognostic indicators of clinical outcomes and immunotherapy responses in patients with TNBC. These findings provide valuable insights and novel avenues for developing immunotherapeutic approaches targeting TNBC.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>581</startPage>
	      	<endPage>593</endPage>
	      	<author>
				Nani Li, Xiaoting Qiu, Jingsong Xue, Limu Yi, Mulan Chen, Zhijian Huang
	      	</author>
	    </item>
    	    <item>
	       	<title>Knockout of &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt; in Rats Alleviates Concanavalin A-induced Autoimmune Hepatitis by Regulating Macrophage Polarization</title>
	      	<link>//article/id/2a57d10b-b489-4995-94ac-ae1fa809788c</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;The effect of the functionally unknown gene &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt; on autoimmune hepatitis was investigated on &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt; knockout rats (&amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt;&amp;lt;sup&amp;lt;&amp;lt;i&amp;lt;-/-&amp;lt;/i&amp;lt;&amp;lt;/sup&amp;lt;) and THP-1 cells.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Method &amp;lt;/b&amp;lt; &amp;nbsp;Six–eight-week-old &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt;&amp;lt;sup&amp;lt;&amp;lt;i&amp;lt;-/-&amp;lt;/i&amp;lt;&amp;lt;/sup&amp;lt; and wild-type (WT) SD rats were injected with Con A (16 mg/kg), and euthanized after 24 h. The sera, livers, and spleens were collected. THP-1 cells and the recombinant protein (rC6ORF120) were used to explore the mechanism &amp;lt;i&amp;lt;in vitro&amp;lt;/i&amp;lt;. The frequency of M1 and M2 macrophages was analyzed using flow cytometry. Western blotting and PCR were used to detect macrophage polarization-associated factors.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;&amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt; knockout attenuated Con A-induced autoimmune hepatitis. Flow cytometry indicated that the proportion of CD68&amp;lt;sup&amp;lt;+&amp;lt;/sup&amp;lt;CD86&amp;lt;sup&amp;lt;+&amp;lt;/sup&amp;lt;M1 macrophages from the liver and spleen in the &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt;&amp;lt;sup&amp;lt;&amp;lt;i&amp;lt;-/-&amp;lt;/i&amp;lt;&amp;lt;/sup&amp;lt; rats decreased. &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt; knockout induced downregulation of CD86 protein and the mRNA levels of related inflammatory factors TNF-α, IL-1β, and IL-6 in the liver. &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt; knockout did not affect the polarization of THP-1 cells. However, rC6ORF120 promoted the THP-1 cells toward CD68&amp;lt;sup&amp;lt;+&amp;lt;/sup&amp;lt;CD80&amp;lt;sup&amp;lt;+&amp;lt;/sup&amp;lt;M1 macrophages and inhibited the CD68&amp;lt;sup&amp;lt;+&amp;lt;/sup&amp;lt;CD206&amp;lt;sup&amp;lt;+&amp;lt;/sup&amp;lt;M2 phenotype.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;&amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt; knockout alleviates Con A-induced autoimmune hepatitis by inhibiting macrophage polarization toward M1 macrophages and reducing the expression of related inflammatory factors in &amp;lt;i&amp;lt;C6orf120&amp;lt;/i&amp;lt;&amp;lt;sup&amp;lt;&amp;lt;i&amp;lt;-/-&amp;lt;/i&amp;lt;&amp;lt;/sup&amp;lt; rats.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>594</startPage>
	      	<endPage>606</endPage>
	      	<author>
				Xin Wang, Yuqi Wang, Hui Liu, Yingying Lin, Peng Wang, Yunyun Yi, Xin Li
	      	</author>
	    </item>
    	    <item>
	       	<title>The Association between GLP-1 Receptor-Based Agonists and the Incidence of Asthma in Patients with Type 2 Diabetes and/or Obesity: A Meta-Analysis</title>
	      	<link>//article/id/0a6d46ba-9014-465f-bb69-79f6d2fc53dd</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;Recent studies have indicated potential anti-inflammatory effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) on asthma, which is often comorbid with type 2 diabetes mellitus (T2DM) and obesity. Therefore, we conducted a meta-analysis to assess the association between the administration of glucagon-like peptide-1 (GLP-1) receptor-based agonists and the incidence of asthma in patients with T2DM and/or obesity.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;PubMed, Web of Science, Embase, the Cochrane Central Register of Controlled Trials, and &amp;lt;i&amp;lt;Clinicaltrial.gov&amp;lt;/i&amp;lt; were systematically searched from inception to July 2023. Randomized controlled trials (RCTs) of GLP-1 receptor-based agonists (GLP-1RA, GLP-1 based dual and triple receptor agonist) with reports of asthma events were included. Outcomes were computed as risk ratios (&amp;lt;i&amp;lt;RR&amp;lt;/i&amp;lt;) using a fixed-effects model.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Overall, 39 RCTs with a total of 85,755 participants were included. Compared to non-GLP-1 receptor-based agonist users, a trend of reduced risk of asthma was observed in patients with T2DM or obesity using GLP-1 receptor-based agonist treatments, although the difference was not statistically significant [&amp;lt;i&amp;lt;RR&amp;lt;/i&amp;lt; = 0.91, 95% confidence interval (&amp;lt;i&amp;lt;CI&amp;lt;/i&amp;lt;): 0.68 to 1.24]. Further Subgroup analyses indicated that the use of light-molecular-weight GLP-1RAs might be associated with a reduced the risk of asthma when compared with non-users (&amp;lt;i&amp;lt;RR&amp;lt;/i&amp;lt; = 0.65, 95% &amp;lt;i&amp;lt;CI&amp;lt;/i&amp;lt;: 0.43 to 0.99, &amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; = 0.043). We also performed sensitivity analyses for participant characteristics, study design, drug structure, duration of action, and drug subtypes. However, no significant associations were observed.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;Compared with non-users, a modest reduction in the incidence of asthma was observed in patients with T2DM or obesity using GLP-1 receptor-based agonist treatments. Further investigations are warranted to assess the association between GLP-1 receptor-based agonists and the risk of asthma.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>607</startPage>
	      	<endPage>616</endPage>
	      	<author>
				Mengqing Zhang, Chu Lin, Xiaoling Cai, Ruoyang Jiao, Shuzhen Bai, Zonglin Li, Suiyuan Hu, Fang Lyu, Wenjia Yang, Linong Ji
	      	</author>
	    </item>
    	    <item>
	       	<title>SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt; Induces Iron Overload and Ferroptosis in Cardiomyocytes in a Silicosis Mouse Model</title>
	      	<link>//article/id/b1a5ce98-cbd7-4768-89f1-9af916f2ab2e</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt;  &amp;nbsp; The aim of this study was to explore the role and mechanism of ferroptosis in SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;-induced cardiac injury using a mouse model.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt;  &amp;nbsp; Male C57BL/6 mice were intratracheally instilled with SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt; to create a silicosis model. Ferrostatin-1 (Fer-1) and deferoxamine (DFO) were used to suppress ferroptosis. Serum biomarkers, oxidative stress markers, histopathology, iron content, and the expression of ferroptosis-related proteins were assessed.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt;  &amp;nbsp; SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt; altered serum cardiac injury biomarkers, oxidative stress, iron accumulation, and ferroptosis markers in myocardial tissue. Fer-1 and DFO reduced lipid peroxidation and iron overload, and alleviated SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;-induced mitochondrial damage and myocardial injury. SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt; inhibited Nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream antioxidant genes, while Fer-1 more potently reactivated Nrf2 compared to DFO.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt;  &amp;nbsp; Iron overload-induced ferroptosis contributes to SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;-induced cardiac injury. Targeting ferroptosis by reducing iron accumulation or inhibiting lipid peroxidation protects against SiO&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt; cardiotoxicity, potentially &amp;lt;i&amp;lt;via&amp;lt;/i&amp;lt; modulation of the Nrf2 pathway.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>617</startPage>
	      	<endPage>627</endPage>
	      	<author>
				Yongheng Wang, Ning Li, Yi Guan, Tong LI, Yuxiu Zhang, Hong Cao, Zhihua Yu, Zhiheng Li, Shuoyan Li, Jiahao Hu, Wenxin Zhou, Sisi Qin, Shuang Li, Sanqiao Yao
	      	</author>
	    </item>
    	    <item>
	       	<title>Antibody Levels and Infection Status of Pertussis in the Population under Pertussis Resurgence in Guangxi in 2018: A Cross-Sectional Survey</title>
	      	<link>//article/id/829bfc43-6a66-4f31-b1eb-6bf542ea4e78</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;Pertussis cases have increased markedly since 2018 in Guangxi. The aim of this study was to evaluate antibody levels and the infection status of pertussis in the resident population.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Method&amp;lt;/b&amp;lt; &amp;nbsp;A total of 10,215 serum samples from residents were collected from August–November 2018 and tested for anti-pertussis IgG and toxin IgG using the enzyme-linked immunosorbent assay (ELISA).&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Of the collected samples, 1,833 (17.94%) tested positive for anti-pertussis IgG, with the median concentration of 16.06 IU/mL. Antibody level &amp;lt; 10 IU/mL accounted for more than 60% in children under 4 years of age, but declined with age, whereas the percentages of the other three levels (10–40, 40–50, and ≥ 50 IU/mL) increased almost with age (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.001). Moreover, 7,924 samples were selected for anti-pertussis toxin IgG, of which 653 (8.24%) tested positive (≥ 40 IU/mL) with the median concentration of 5.89 IU/mL, and 204 participants (2.56%) had recent pertussis infection (≥ 100 IU/mL). Among the different age groups, the highest rates of positivity and recent infection were observed at 11–20 years of age, the lowest positivity rate at 5 years of age, and the lowest recent infection rate at 4 years of age (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.001, &amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; = 0.005, respectively). &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion &amp;lt;/b&amp;lt; &amp;nbsp;The survey results showed that all age groups in Guangxi lacked immunity against pertussis, which was one of the main factors contributing to the resurgence of pertussis in 2018. In addition, the prevalence of pertussis is relatively high in Guangxi, and its incidence is seriously underestimated, especially in adolescents and adults.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>628</startPage>
	      	<endPage>638</endPage>
	      	<author>
				Liang Liang, Qiuyun Deng, Lili Deng, Jinghang Wei, Shiyi Chen, Yizhi Wei, Yuyan Ma, Yue Qin, Wei Liu
	      	</author>
	    </item>
    	    <item>
	       	<title>Development of an Integrated Disposable Device for SARS-CoV-2 Nucleic Acid Extraction and Detection</title>
	      	<link>//article/id/4f2eb9ed-8724-4847-b0f7-7cd00e50b814</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp; To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp; We designed, developed, and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and detection. The precision of the liquid transfer and temperature control was tested. A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). The entire process, from SARS-CoV-2 nucleic acid extraction to amplification, was evaluated.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp; The precision of the syringe transfer volume was 19.2 ± 1.9 μL (set value was 20), 32.2 ± 1.6 (set value was 30), and 57.2 ± 3.5 (set value was 60). Temperature control in the amplification tube was measured at 60.0 ± 0.0 °C (set value was 60) and 95.1 ± 0.2 °C (set value was 95) respectively. SARS-Cov-2 nucleic acid extraction yield through the device was 7.10 × 10&amp;lt;sup&amp;lt;6&amp;lt;/sup&amp;lt; copies/mL, while a commercial kit yielded 2.98 × 10&amp;lt;sup&amp;lt;6&amp;lt;/sup&amp;lt; copies/mL. The mean time to complete the entire assay, from SARS-CoV-2 nucleic acid extraction to amplification detection, was 36 min and 45 s. The detection limit for SARS-CoV-2 nucleic acid was 250 copies/mL.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp; The integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test (POCT).&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>639</startPage>
	      	<endPage>646</endPage>
	      	<author>
				Jing Ma, Yanzhe Hao, Meiling Hou, Xiaoshan Zhang, Jingduan Liu, Haodi Meng, Jiangbo Chang, Xuejun Ma, Jihua Liu, Qingjie Ying, Xianhua Wang, Hongxia Li, Yuxi Cao, Xiaoguang Zhang
	      	</author>
	    </item>
    	    <item>
	       	<title>Research Progress on the Association between Schizophrenia and &amp;lt;i&amp;lt;Toxoplasma gondii&amp;lt;/i&amp;lt; Infection</title>
	      	<link>//article/id/c2232b9d-f8de-403d-8161-b144a4f212d6</link>
	     	<description><![CDATA[&amp;lt;i&amp;lt;Abstract: Toxoplasma gondii &amp;lt;/i&amp;lt;(&amp;lt;i&amp;lt;T. gondii or Tg&amp;lt;/i&amp;lt;), is an obligatory intracellular parasite with humans as its intermediate hosts. In recent years, significant correlations between &amp;lt;i&amp;lt;T. gondii&amp;lt;/i&amp;lt; infection and schizophrenia have been reported, including the possible mediating mechanisms. Currently, mechanisms and hypotheses focus on central neurotransmitters, immunity, neuroinflammation, and epigenetics; however, the exact underlying mechanisms remain unclear. In this article, we review the studies related to &amp;lt;i&amp;lt;T. gondii&amp;lt;/i&amp;lt; infection and schizophrenia, particularly the latest research progress. Research on dopamine (DA) and other neurotransmitters, the blood-brain barrier, inflammatory factors, disease heterogeneity, and other confounders is also discussed. In addition, we also summarized the results of some new epidemiological investigations.]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>647</startPage>
	      	<endPage>660</endPage>
	      	<author>
				Yiting Zhu, Xiaohui Yang, Miaoru Chen, Yu Hu, Yunfeng Chang, Xiang Wu
	      	</author>
	    </item>
    	    <item>
	       	<title>Selenium, Type-2 Diabetes, and the Possible Protective Role of Vitamin D</title>
	      	<link>//article/id/da39b3ed-f8b1-42f9-8d1c-35685e736146</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>661</startPage>
	      	<endPage>665</endPage>
	      	<author>
				Runxue Ma, Ce Liu, Ling Zhang, Jingzhe Guo, Erkai Zhou, Ling Zheng, Li He, Xiaobing Shan, Yunhui Yu, Bin Luo
	      	</author>
	    </item>
    	    <item>
	       	<title>Associations of Perchlorate, Nitrate, and Thiocyanate with Sex Hormones among Children: A Nationally Representative Cross-sectional Study in U.S.</title>
	      	<link>//article/id/1c573e84-da3d-47fb-b9dc-f5404c98a646</link>
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	      	<issue>6</issue>
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	      	<endPage>671</endPage>
	      	<author>
				Yiwen Wang, Guodong Ding, Peipei Hu, Yongjun Zhang
	      	</author>
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    	    <item>
	       	<title>Genetically Predicted Plasma Levels of Amino Acids and Endometriosis: A Mendelian Randomization Study</title>
	      	<link>//article/id/4d1ecad3-0e03-4ce2-93ca-3e1c1086bdcf</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>672</startPage>
	      	<endPage>675</endPage>
	      	<author>
				Juanmei Li, Yao Ni, Ning Wang, Yu Liao, Yiwei Yu, Wenliang Lyu, Ruihua Zhao
	      	</author>
	    </item>
    	    <item>
	       	<title>Inhibition of the NF-κB Signaling Pathway Improves Cigarette Mainstream Smoke-Induced Lung Injury and Gut Microbiota Disturbance</title>
	      	<link>//article/id/92688fca-5f58-427e-b952-dbd256e2fa19</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>676</startPage>
	      	<endPage>681</endPage>
	      	<author>
				Hong Huang, Shengjie Li, Lifang Zeng, Yan Zhang, Ying Chen, Yanbing Ma, Jing Wei, Changwei Zou, Tingtao Chen
	      	</author>
	    </item>
    	    <item>
	       	<title>Health Effects of Extreme Low Temperatures and Cold Waves on Respiratory Diseases</title>
	      	<link>//article/id/7ff17134-5337-4ca5-a84a-56dc044d9e09</link>
	     	<description><![CDATA[]]></description>
	      	<volume>37</volume>
	      	<issue>6</issue>
	      	<startPage>682</startPage>
	      	<endPage>685</endPage>
	      	<author>
				Zongrui Liu, Yuxia Ma, Yuhan Zhao, Wanci Wang, Pengpeng Qin, Jie Yang, Bowen Cheng
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