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      <title>Biomedical and Environmental Sciences</title>
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    <description><![CDATA[《Biomedical and Environmental Sciences》2022年第9期]]></description>
    <year><![CDATA[2022]]></year>
    <volume><![CDATA[35]]></volume>
    <issue><![CDATA[9]]></issue>
    	    <item>
	       	<title>Cover</title>
	      	<link>//article/id/a2414fed-6531-445a-96cb-279ebfc3cb81</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage></startPage>
	      	<endPage></endPage>
	      	<author>
				
	      	</author>
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    	    <item>
	       	<title>Contents</title>
	      	<link>//article/id/d81dd69b-904f-4d43-af01-0111cb2c8889</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>1</startPage>
	      	<endPage>2</endPage>
	      	<author>
				
	      	</author>
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    	    <item>
	       	<title>Key Social Determinants to Narrow the Gap between Health-adjusted Life Expectancy and Life Expectancy in Megacities</title>
	      	<link>//article/id/5a594266-aa29-42f7-aab4-87b5a41eb2a3</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;Improvement in the quality of life is reflected in the narrowing of the gap between health-adjusted life expectancy (HALE) and life expectancy (LE). The effect of megacity expansion on narrowing the gap is rarely reported. This study aimed to disclose this potential relationship.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Annual life tables were constructed from identified death records and population counts from multiple administrative sources in Guangzhou, China, from 2010 to 2020. Joinpoint regression was used to evaluate the temporal trend. Generalized principal component analysis and multilevel models were applied to examine the county-level association between the gap and social determinants.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Although LE and HALE in megacities are increasing steadily, their gap is widening. Socio-economic and health services are guaranteed to narrow this gap. Increasing personal wealth, a growing number of newborns and healthy immigrants, high urbanization, and healthy aging have helped in narrowing this gap.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;In megacities, parallel LE and HALE growth should be highly considered to narrow their gap. Multiple social determinants need to be integrated as a whole to formulate public health plans.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>773</startPage>
	      	<endPage>781</endPage>
	      	<author>
				XI Jun Yan, CHEN Yuan Yuan, ZHANG Yu Qin, LUO Ao, DU Zhi Cheng, LIANG Bo Heng, DONG Hang, LIN Xiao, QIN Peng Zhe, HAO Yuan Tao
	      	</author>
	    </item>
    	    <item>
	       	<title>Rabies Virus Neutralizing Activity, Safety, and Immunogenicity of Recombinant Human Rabies Antibody Compared with Human Rabies Immunoglobulin in Healthy Adults</title>
	      	<link>//article/id/f52ccc41-bb11-4a18-946d-f2745336f391</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;Preliminary assessment of rabies virus neutralizing activity, safety and immunogenicity of a recombinant human rabies antibody (NM57) compared with human rabies immunoglobulin (HRIG) in Chinese healthy adults.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Subjects were randomly (1:1:1) allocated to Groups A (20 IU/kg NM57), B (40 IU/kg NM57), or C (20 IU/kg HRIG). One injection was given on the day of enrollment. Blood samples were collected on days −7 to 0 (pre-injection), 3, 7, 14, 28, and 42. Adverse events (AEs) and serious AEs (SAEs) were recorded over a period of 42 days after injection.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;All 60 subjects developed detectable rabies virus neutralizing antibodies (RVNAs) (&amp;gt; 0.05 IU/mL) on days 3, 7, 14, 28, and 42. The RVNA levels peaked on day 3 in all three groups, with a geometric mean concentration (GMC) of 0.2139 IU/mL in Group A, 0.3660 IU/mL in Group B, and 0.1994 IU/mL in Group C. At each follow-up point, the GMC in Group B was significantly higher than that in Groups A and C. The areas under the antibody concentration curve over 0–14 days and 0–42 days in Group B were significantly larger than those in Groups A and C. Fifteen AEs were reported. Except for one grade 2 myalgia in Group C, the other 14 were all grade 1. No SAEs were observed.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;The rabies virus neutralizing activity of 40 IU/kg NM57 was superior to that of 20 IU/kg NM57 and 20 IU/kg HRIG, and the rabies virus neutralizing activity of 20 IU/kg NM57 and 20 IU/kg HRIG were similar. Safety was comparable between NM57 and HRIG.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>782</startPage>
	      	<endPage>791</endPage>
	      	<author>
				ZHANG Jun Nan, MENG Ya Juan, BAI Yun Hua, LI Yu Feng, YANG Li Qing, SHI Nian Min, HAN Hui Xia, GAO Jian, ZHU Li Juan, LI Shu Ping, ZHANG Jing, ZHAO Qin Hua, WANG Xiu Qin, WEI Jing Shuang, REN Le Min, CAO Chen Hua, CHEN Chen, ZHAO Wei, LI Li
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    	    <item>
	       	<title>Montanide ISA-720 and Naloxone in HBsAg Vaccine Formulation: Cytokine Profiling and Monitoring of Long-Lasting Humoral Immune Responses</title>
	      	<link>//article/id/d74ec60e-a653-4b45-bde3-1aa1d9f7d3d8</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;This study aimed to investigate the effects of Montanide ISA-720 and Naloxone (NLX) in Hepatitis B surface antigen (HBsAg) vaccine formulation on cytokine and long-lasting antibody responses.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;First, the HBsAg was formulated in Montanide ISA-720 adjuvant and Naloxone at 5 and 10 mg/kg. The experimental mice were immunized three times at a 2-week interval, and then IL-4, IL-2, TNF-α, and IFN-γ cytokines; long-lasting IgG antibody responses 220 days after the last shot; and IgG1/IgG2a isotypes were assessed by ELISA.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;The HBsAg-Alum group exhibited the highest IL-4 cytokine response among the experimental groups, whereas NLX in HBsAg-MON720 vaccine formulation did not affect cytokine responses. In addition, NLX in Alum-based vaccine suppressed IL-4 cytokine response and increased the IL-2/IL-4 cytokine ratio. Moreover, HBsAg-MON720 was more potent than HBsAg-Alum in the induction of antibody responses, and NLX in Alum- and MON720-based vaccines induced long-lasting antibody responses.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;NLX in Alum-based vaccine decreased IL-4 cytokine response, increased IL-2/IL-4 cytokine ratio, and improved long-lasting humoral immune responses in both vaccine formulations. Therefore, the adjuvant activity of NLX in the vaccine formulation depends on the type of adjuvant and the nature of the antigen in the vaccine formulation.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>792</startPage>
	      	<endPage>803</endPage>
	      	<author>
				Mina Mirzaee, Setareh Haghighat, Bahareh Golkaran, Fatemeh Asgarhalvaei, Rayhaneh Mirzaee, Morteza Taghizadeh, Mohammad Ali Savoji, Behzad Esfandiari, Mehdi Mahdavi
	      	</author>
	    </item>
    	    <item>
	       	<title>The Viral Load of Epstein-Barr Virus in Blood of Children after Hematopoietic Stem Cell Transplantation</title>
	      	<link>//article/id/7135f898-1779-41c1-8b6f-620e70c512ff</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;To detect the Epstein-Barr virus (EBV) viral load of children after hematopoietic stem cell transplantation (HSCT) using chip digital PCR (cdPCR). &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;The sensitivity of cdPCR was determined using EBV plasmids and the EBV B95-8 strain. The specificity of EBV cdPCR was evaluated using the EBV B95-8 strain and other herpesviruses (herpes simplex virus 1, herpes simplex virus 2, varicella zoster virus, human cytomegalovirus, human herpesvirus 6, and human herpesvirus 7). From May 2019 to September 2020, 64 serum samples of children following HSCT were collected. EBV infection and the viral load of serum samples were detected by cdPCR. The epidemiological characteristics of EBV infections were analyzed in HSCT patients. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;The limit of detection of EBV cdPCR was 110 copies/mL, and the limit of detection of EBV quantitative PCR was 327 copies/mL for the pUC57-BALF5 plasmid. The result of EBV cdPCR was up to 121 copies/mL in the EBV B95-8 strain, and both were more sensitive than that of quantitative PCR. Using cdPCR, the incidence of EBV infection was 18.75% in 64 children after HSCT. The minimum EBV viral load was 140 copies/mL, and the maximum viral load was 3,209 copies/mL using cdPCR. The average hospital stay of children with EBV infection (184 ± 91 days) was longer than that of children without EBV infection (125 ± 79 days), &amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; = 0.026. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;EBV cdPCR had good sensitivity and specificity. The incidence of EBV infection was 18.75% in 64 children after HSCT from May 2019 to September 2020. EBV cdPCR could therefore be a novel method to detect EBV viral load in children after HSCT.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>804</startPage>
	      	<endPage>810</endPage>
	      	<author>
				WANG Wen Jun, FENG Shun Qiao, HE Feng, DU Hai Jun, FENG Miao, WANG Rui Fang, MEI Guo Yong, LIU Mi, LIU Rong, YAO Hai Lan, HAN Jun
	      	</author>
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    	    <item>
	       	<title>Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhance the Osteoblastic Differentiation of Periodontal Ligament Stem Cells Under High Glucose Conditions Through the PI3K/AKT Signaling Pathway</title>
	      	<link>//article/id/2de0a461-2be2-4953-99cc-198c3ca800f1</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;High glucose (HG) can influence the osteogenic differentiation ability of periodontal ligament stem cells (PDLSCs). Human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-exo) have broad application prospects in tissue healing. The current study aimed to explore whether hUCMSC-exo could promote the osteogenic differentiation of hPDLSCs under HG conditions and the underlying mechanism.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp; We used a 30 mmol/L glucose concentration to simulate HG conditions. CCK-8 assay was performed to evaluate the effect of hUCMSC-exo on the proliferation of hPDLSCs. Alkaline phosphatase (ALP) staining, ALP activity, and qRT-PCR were performed to evaluate the pro-osteogenic effect of hUCMSC-exo on hPDLSCs. Western blot analysis was conducted to evaluate the underlying mechanism.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp; The results of the CCK-8 assay, ALP staining, ALP activity, and qRT-PCR assay showed that hUCMSC-exo significantly promoted cell proliferation and osteogenic differentiation in a dose-dependent manner. The Western blot results revealed that hUCMSC-exo significantly increased the levels of p-PI3K and p-AKT in cells, and the effect was inhibited by LY294002 (PI3K inhibitor) or MK2206 (AKT inhibitor), respectively. Moreover, the increases in osteogenic indicators induced by hUCMSC-exo were significantly suppressed by LY294002 and MK2206.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp; hUCMSC-exo promote the osteogenic differentiation of hPDLSCs under HG conditions through the PI3K/AKT signaling pathway.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>811</startPage>
	      	<endPage>820</endPage>
	      	<author>
				YANG Shuo, ZHU Biao, TIAN Xiao Yu, YU Han Ying, QIAO Bo, ZHAO Li Sheng, ZHANG Bin
	      	</author>
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    	    <item>
	       	<title>H-NS Represses Biofilm Formation and c-di-GMP Synthesis in &amp;lt;i&amp;lt;Vibrio parahaemolyticus&amp;lt;/i&amp;lt;</title>
	      	<link>//article/id/64150be1-6cd8-4bdc-b3ac-c644dd47b4e0</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in &amp;lt;i&amp;lt;Vibrio parahaemolyticus&amp;lt;/i&amp;lt; RIMD2210633.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;The deletion of &amp;lt;i&amp;lt;hns&amp;lt;/i&amp;lt; enhanced the biofilm formation and intracellular c-di-GMP levels in &amp;lt;i&amp;lt;V. parahaemolyticus&amp;lt;/i&amp;lt; RIMD2210633. H-NS can bind the upstream promoter–proximal DNA regions of &amp;lt;i&amp;lt;scrA&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;scrG&amp;lt;/i&amp;lt;, VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;One of the mechanisms by which H-NS represses the biofilm formation by&amp;lt;i&amp;lt; V. parahaemolyticus&amp;lt;/i&amp;lt; RIMD2210633 may be &amp;lt;i&amp;lt;via&amp;lt;/i&amp;lt; repression of the production of intracellular c-di-GMP.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>821</startPage>
	      	<endPage>829</endPage>
	      	<author>
				XUE Xing Fan, ZHNAG Miao Miao, SUN Jun Fang, LI Xue, WU Qi Min, YIN Zhe, YANG Wen Hui, NI Bin, HU Ling Fei, ZHOU Dong Sheng, LU Ren Fei, ZHANG Yi Quan
	      	</author>
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    	    <item>
	       	<title>Effect of Maximal Oxygen Pulse on Patients with Chronic Obstructive Pulmonary Disease</title>
	      	<link>//article/id/d41c81c5-e15a-4a4a-a13a-55f1f0a50434</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;This study evaluated the effect of maximal oxygen pulse (O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt;) on patients with chronic obstructive pulmonary disease (COPD) and confirmed the predictive effect on acute exacerbations of COPD (AECOPD).&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;This retrospective study included 91 participants who underwent cardiopulmonary exercise testing (CPET), lung function testing, a dyspnea scale assessment, and a 3-year follow-up. The participants were divided into two groups according to the O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt; value. Exercise capacity, ventilatory conditions, gas exchange efficiency, and dyspnea symptoms were compared, and the correlations between O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt; and these indices were evaluated. The ability of O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt; to predict AECOPD was examined.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Exercise capacity, ventilatory conditions, and gas exchange efficiency were lower, and dyspnea symptom scores were higher in the impaired O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt; group (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.05). O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt; was positively correlated with forced vital capacity (FVC)%, forced expiratory volume in 1 sec (FEV&amp;lt;sub&amp;lt;1&amp;lt;/sub&amp;lt;)%, FEV&amp;lt;sub&amp;lt;1&amp;lt;/sub&amp;lt;/FVC%, anaerobic threshold (AT), work rate (WR)%, aximal oxygen uptake (V̇O&amp;lt;sub&amp;lt;2max&amp;lt;/sub&amp;lt;)%, V̇O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;/kg&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt;, V̇O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;/kg&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt;%, WR&amp;lt;sub&amp;lt;AT&amp;lt;/sub&amp;lt;, WR&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt;, V̇O&amp;lt;sub&amp;lt;2AT&amp;lt;/sub&amp;lt;, V̇O&amp;lt;sub&amp;lt;2max&amp;lt;/sub&amp;lt;, and V̇&amp;lt;sub&amp;lt;Emax&amp;lt;/sub&amp;lt;, and was negatively correlated with EqCO&amp;lt;sub&amp;lt;2AT&amp;lt;/sub&amp;lt;, and EqCO&amp;lt;sub&amp;lt;2max&amp;lt;/sub&amp;lt; (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.05). Most importantly, O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt; could be used to predict AECOPD, and the best cut-off value was 89.5% (area under the curve, 0.739; 95% &amp;lt;i&amp;lt;CI&amp;lt;/i&amp;lt;, 0.609–0.869).&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;O&amp;lt;sub&amp;lt;2&amp;lt;/sub&amp;lt;P&amp;lt;sub&amp;lt;max&amp;lt;/sub&amp;lt; reflected exercise capacity, ventilation capacity, gas exchange capacity, and dyspnea symptoms in patients with COPD and may be an independent predictor of AECOPD.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>830</startPage>
	      	<endPage>841</endPage>
	      	<author>
				LI Yun Xiao, WANG Jun, WU Bo, LIN Fang, TAN Chun Ting, YU Gang Gang, NIE Shan, ZHAO Ran Ran, XU Bo
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	       	<title>Genetic Diversity, Antibiotic Resistance, and Pathogenicity of &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; Species from Food Products in Shanghai, China</title>
	      	<link>//article/id/22808ff7-0b7d-4889-9254-509566f79741</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;&amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; has recently been recognized as an emerging human pathogen. &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt;-associated diarrhea is a phenomenon occurring worldwide. This study was designed to determine the prevalence, genetic diversity, antibiotic resistance, and pathogenicity of &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; strains isolated from food products in Shanghai.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;&amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; isolates (&amp;lt;i&amp;lt;n&amp;lt;/i&amp;lt; = 79) collected from food samples were analyzed using concatenated &amp;lt;i&amp;lt;gyrB&amp;lt;/i&amp;lt;-&amp;lt;i&amp;lt;cpn60&amp;lt;/i&amp;lt; sequencing. The antibiotic resistance of these isolates was determined using antimicrobial susceptibility testing. Pathogenicity was assessed using β-hemolytic, extracellular protease, virulence gene detection, &amp;lt;i&amp;lt;C. elegans&amp;lt;/i&amp;lt; liquid toxicity (LT), and cytotoxicity assays.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Eight different species were identified among the 79 isolates. The most prevalent &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; species were &amp;lt;i&amp;lt;A. veronii&amp;lt;/i&amp;lt; [62 (78.5%)], &amp;lt;i&amp;lt;A. caviae&amp;lt;/i&amp;lt; [6 (7.6%)], &amp;lt;i&amp;lt;A. dhakensis&amp;lt;/i&amp;lt; [3 (3.8%)], and &amp;lt;i&amp;lt;A. salmonicida&amp;lt;/i&amp;lt; [3 (3.8%)]. The &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; isolates were divided into 73 sequence types (STs), of which 65 were novel. The isolates were hemolytic (45.6%) and protease-positive (81.0%). The most prevalent virulence genes were &amp;lt;i&amp;lt;act&amp;lt;/i&amp;lt; (73.4%), &amp;lt;i&amp;lt;fla&amp;lt;/i&amp;lt; (69.6%), &amp;lt;i&amp;lt;aexT&amp;lt;/i&amp;lt; (36.7%), and &amp;lt;i&amp;lt;ascV&amp;lt;/i&amp;lt; (30.4%). The results of &amp;lt;i&amp;lt;C. elegans&amp;lt;/i&amp;lt; LT and cytotoxicity assays revealed that &amp;lt;i&amp;lt;A. dhakensis&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;A. hydrophila&amp;lt;/i&amp;lt; were more virulent than &amp;lt;i&amp;lt;A. veronii&amp;lt;/i&amp;lt;,&amp;lt;i&amp;lt; A. caviae&amp;lt;/i&amp;lt;, and &amp;lt;i&amp;lt;A. bivalvium&amp;lt;/i&amp;lt;. Antibiotic resistance genes [&amp;lt;i&amp;lt;tetE&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;blaTEM&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;tetA&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;qnrS&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;aac(6)-Ib&amp;lt;/i&amp;lt;, mcr&amp;lt;i&amp;lt;-1&amp;lt;/i&amp;lt;, and &amp;lt;i&amp;lt;mcr-3&amp;lt;/i&amp;lt;] were detected in the isolates. The multidrug-resistance rate of the &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; isolates was 11.4%, and 93.7% of the &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; isolates were resistant to cefazolin.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;The taxonomy, antibiotic resistance, and pathogenicity of different &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; species varied. The &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; isolates &amp;lt;i&amp;lt;A. dhakensis&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;A. hydrophila&amp;lt;/i&amp;lt; were highly pathogenic, indicating that food-derived &amp;lt;i&amp;lt;Aeromonas&amp;lt;/i&amp;lt; isolates are potential risks for public health and food safety. The monitoring of food quality and safety will result in better prevention and treatment strategies to control diarrhea illnesses in China.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>842</startPage>
	      	<endPage>853</endPage>
	      	<author>
				QU Feng Tian, WANG Wen Qing, LIU Qian, ZHOU Hai Jian, HU Jin Rui, DU Xiao Li, WANG Yue, XUE Jia Qi, CUI Zhi Gang, XIE Gui Lin, MENG Shuang
	      	</author>
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	       	<title>Construction of MicroRNA-Target Interaction Networks Based on MicroRNA Expression Profiles of HRV16-infected H1-HeLa Cells</title>
	      	<link>//article/id/7854539c-da08-4ca1-9dc0-df75f02604bc</link>
	     	<description><![CDATA[In the present study we investigated the changes in miRNA levels inhuman rhinovirus 16 (HRV16)-infected cells. A small RNA deep sequencing experiment was performed through next-generation sequencing. In total, 53 differentially expressed miRNAs were confirmed by RT-qPCR, including 37 known miRNAs and 16 novel miRNAs. Interaction networks between differentially expressed miRNAs and their targets were established by mirDIP and Navigator. The prediction results showed that &amp;lt;i&amp;lt;QKI&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;NFAT5&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;BNC2&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;CELF2&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;LCOR&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;MBNL2&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;MTMR3&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;NFIB&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;PPARGC1A&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;RSBN1&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;TRPS1&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;WDR26&amp;lt;/i&amp;lt;, and &amp;lt;i&amp;lt;ZNF148&amp;lt;/i&amp;lt;, which are associated with cellular differentiation and transcriptional regulation, were recognized by 12, 11, or 9 miRNAs. Many correlations were observed between transcriptional or post-transcriptional regulation of an miRNA and the expression levels of its target genes in HRV16-infected H1-HeLa cells.]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>854</startPage>
	      	<endPage>860</endPage>
	      	<author>
				SONG Qin Qin, WANG Yan Hai, WANG Xin Ling, SHI Bing Tian, WANG Rui Fang, SONG Juan, WANG Wen Jun, XIA Dong, XIA Zhi Qiang, WEI Qiang, HAN Jun
	      	</author>
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	       	<title>Evaluating the Quality of Case-control Studies involving the Association between Tobacco Exposure and Diseases in a Chinese Population based on the Newcastle-Ottawa Scale and &amp;lt;i&amp;lt;Post-hoc&amp;lt;/i&amp;lt; Power</title>
	      	<link>//article/id/27378cac-9e6d-426c-a6e2-aa24a99fd0b7</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>861</startPage>
	      	<endPage>866</endPage>
	      	<author>
				SHI Yu Lin, ZHAO Jian, AI Fei Ling, WANG Yu Tong, HU Kui Ru, WANG Xue Wei, YANG Wen Yi, WANG Jing Xin, AI Li Mei, WAN Xia
	      	</author>
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	       	<title>Soft Tissue Myoepithelioma in Children: A Rare Case Report of the Mediastinum and a Review of the Literature</title>
	      	<link>//article/id/cf239966-06aa-4932-b0a9-c5f0e90c5dc4</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>867</startPage>
	      	<endPage>871</endPage>
	      	<author>
				DU Hong Mei, CAI Wei Song
	      	</author>
	    </item>
    	    <item>
	       	<title>Exercise for Health Suzhou Initiative</title>
	      	<link>//article/id/f6cfd132-dd83-4539-be87-6308d02aba89</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>9</issue>
	      	<startPage>872</startPage>
	      	<endPage>873</endPage>
	      	<author>
				
	      	</author>
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