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      <title>Biomedical and Environmental Sciences</title>
    <link>/</link>
    <description><![CDATA[《Biomedical and Environmental Sciences》2022年第6期]]></description>
    <year><![CDATA[2022]]></year>
    <volume><![CDATA[35]]></volume>
    <issue><![CDATA[6]]></issue>
    	    <item>
	       	<title>Cover</title>
	      	<link>//article/id/654ef03c-6b67-4775-9179-f75eff464fcc</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage></startPage>
	      	<endPage></endPage>
	      	<author>
				
	      	</author>
	    </item>
    	    <item>
	       	<title>Contents</title>
	      	<link>//article/id/a515d658-cc78-459c-ad52-7998e30fb2d2</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>1</startPage>
	      	<endPage>2</endPage>
	      	<author>
				
	      	</author>
	    </item>
    	    <item>
	       	<title>Exosomes from PM&amp;lt;sub&amp;lt;2.5&amp;lt;/sub&amp;lt;-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential</title>
	      	<link>//article/id/4de824a9-43e7-4692-84a7-b79fd402137a</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;Fine particulate matter (PM&amp;lt;sub&amp;lt;2.5&amp;lt;/sub&amp;lt;) is an air pollutant that has become of great concern in recent years. Numerous studies have found that PM&amp;lt;sub&amp;lt;2.5&amp;lt;/sub&amp;lt; may contribute to lung cancer, but the pathogenesis has not yet been fully elucidated. In this study, we explored the roles of exosomes from bronchial epithelial cells in PM&amp;lt;sub&amp;lt;2.5&amp;lt;/sub&amp;lt;-promoted lung cancer metastasis.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Exosomes were isolated from cell supernatants. An animal model of lung metastasis (established by tail vein injection of A549-luc) and &amp;lt;i&amp;lt;in vitro&amp;lt;/i&amp;lt; studies with lung cancer cell lines were used to investigate the effects of exosomes derived from PM&amp;lt;sub&amp;lt;2.5&amp;lt;/sub&amp;lt;-treated human bronchial epithelial cells (PHBE-exo).&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;The animal experiments revealed that PHBE-exo-treated mice showed stronger luciferase activity and a larger relative metastatic region in the lungs, thus indicating that PHBE-exo promoted the metastatic potential of lung cancer. Additionally, PHBE-exo promoted the migration, invasion and epithelial-to-mesenchymal transition of lung cancer cells, in a manner mediated by activation of c-Jun N-terminal kinase.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;These results implied that PM&amp;lt;sub&amp;lt;2.5&amp;lt;/sub&amp;lt; may promote the development of lung cancer through exosomes derived from bronchial epithelial cells, thus providing a potential interventional target for lung cancer. These findings broadened our understanding of cancer-promoting mechanisms of environmental pollutants.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>473</startPage>
	      	<endPage>484</endPage>
	      	<author>
				YU Heng Yi, GUO Hua Qi, FENG Yan, CHENG Wei, WANG Yan
	      	</author>
	    </item>
    	    <item>
	       	<title>MicroRNA-125b Accelerates and Promotes PML-RARa-driven Murine Acute Promyelocytic Leukemia</title>
	      	<link>//article/id/0fb302c7-76a1-41fd-b184-3c322bdbd2c6</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;Most acute promyelocytic leukemia cases are characterized by the PML-RARa fusion oncogene and low white cell counts in peripheral blood.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Based on the frequent overexpression of miR-125-family miRNAs in acute promyelocytic leukemia, we examined the consequence of this phenomenon by using an inducible mouse model overexpressing human miR-125b.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;MiR-125b expression significantly accelerates PML-RARa-induced leukemogenesis, with the resultant induced leukemia being partially dependent on continued miR-125b overexpression. Interestingly, miR-125b expression led to low peripheral white cell counts to bone marrow blast percentage ratio, confirming the clinical observation in acute promyelocytic leukemia patients.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;This study suggests that dysregulated miR-125b expression is actively involved in disease progression and pathophysiology of acute promyelocytic leukemia, indicating that targeting miR-125b may represent a new therapeutic option for acute promyelocytic leukemia.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>485</startPage>
	      	<endPage>493</endPage>
	      	<author>
				GUO Bo, QIN Ran, CHEN Ji Jun, PAN Wen, LU Xue Chun
	      	</author>
	    </item>
    	    <item>
	       	<title>A Novel Early Warning Model for Hand, Foot and Mouth Disease Prediction Based on a Graph Convolutional Network</title>
	      	<link>//article/id/f33f2f1c-dc94-43de-a7c4-48cb83b3682d</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objectives&amp;lt;/b&amp;lt; &amp;nbsp;Hand, foot and mouth disease (HFMD) is a widespread infectious disease that causes a significant disease burden on society. To achieve early intervention and to prevent outbreaks of disease, we propose a novel warning model that can accurately predict the incidence of HFMD.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;We propose a spatial-temporal graph convolutional network (STGCN) that combines spatial factors for surrounding cities with historical incidence over a certain time period to predict the future occurrence of HFMD in Guangdong and Shandong between 2011 and 2019. The 2011–2018 data served as the training and verification set, while data from 2019 served as the prediction set. Six important parameters were selected and verified in this model and the deviation was displayed by the root mean square error and the mean absolute error.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;As the first application using a STGCN for disease forecasting, we succeeded in accurately predicting the incidence of HFMD over a 12-week period at the prefecture level, especially for cities of significant concern.&amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusions&amp;lt;/b&amp;lt; &amp;nbsp;This model provides a novel approach for infectious disease prediction and may help health administrative departments implement effective control measures up to 3 months in advance, which may significantly reduce the morbidity associated with HFMD in the future.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>494</startPage>
	      	<endPage>503</endPage>
	      	<author>
				JI Tian Jiao, CHENG Qiang, ZHANG Yong, ZENG Han Ri, WANG Jian Xing, YANG Guan Yu, XU Wen Bo, LIU Hong Tu
	      	</author>
	    </item>
    	    <item>
	       	<title>20-Hydroxyecdysone Improves Neuronal Differentiation of Adult Hippocampal Neural Stem Cells in High Power Microwave Radiation-Exposed Rats</title>
	      	<link>//article/id/cccb51aa-b68c-469b-ba4d-9c819583241c</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;The hippocampus is thought to be a vulnerable target of microwave exposure. The aim of the present study was to investigate whether 20-hydroxyecdysone (20E) acted as a fate regulator of adult rat hippocampal neural stem cells (NSCs). Furthermore, we investigated if 20E attenuated high power microwave (HMP) radiation-induced learning and memory deficits. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Sixty male Sprague-Dawley rats were randomly divided into three groups: normal controls, radiation treated, and radiation+20E treated. Rats in the radiation and radiation+20E treatment groups were exposed to HPM radiation from a microwave emission system. The learning and memory abilities of the rats were assessed using the Morris water maze test. Primary adult rat hippocampal NSCs were isolated &amp;lt;i&amp;lt;in vitro&amp;lt;/i&amp;lt; and cultured to evaluate their proliferation and differentiation. In addition, hematoxylin &amp; eosin staining, western blotting, and immunofluorescence were used to detect changes in the rat brain and the proliferation and differentiation of the adult rat hippocampal NSCs after HPM radiation exposure. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;The results showed that 20E induced neuronal differentiation of adult hippocampal NSCs from HPM radiation-exposed rats &amp;lt;i&amp;lt;via&amp;lt;/i&amp;lt; the Wnt3a/β-catenin signaling pathway &amp;lt;i&amp;lt;in vitro&amp;lt;/i&amp;lt;. Furthermore, 20E facilitated neurogenesis in the subgranular zone of the rat brain following HPM radiation exposure. Administration of 20E attenuated learning and memory deficits in HPM radiation-exposed rats and frizzled-related protein (FRZB) reduced the 20E-induced nuclear translocation of β-catenin, while FRZB treatment also reversed 20E-induced neuronal differentiation of NSCs &amp;lt;i&amp;lt;in vitro&amp;lt;/i&amp;lt;. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;These results suggested that 20E was a fate regulator of adult rat hippocampal NSCs, where it played a role in attenuating HPM radiation-induced learning and memory deficits.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>504</startPage>
	      	<endPage>517</endPage>
	      	<author>
				LIU Jing Jing, ZHANG Hong Yan, CHEN Xin, ZHANG Guang Bin, LIN Jiang Kai, FENG Hua, CHU Wei Hua
	      	</author>
	    </item>
    	    <item>
	       	<title>Visual Detection of &amp;lt;i&amp;lt;Vibrio parahaemolyticus&amp;lt;/i&amp;lt; using Combined CRISPR/Cas12a and Recombinase Polymerase Amplification</title>
	      	<link>//article/id/c0eac0f1-3125-4814-be04-db99b4a4c325</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;To establish an ultra-sensitive, ultra-fast, visible detection method for &amp;lt;i&amp;lt;Vibrio parahaemolyticus&amp;lt;/i&amp;lt; (VP)&amp;lt;i&amp;lt;.&amp;lt;/i&amp;lt; &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;We established a new method for detecting the &amp;lt;i&amp;lt;tdh&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;trh&amp;lt;/i&amp;lt; genes of VP using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 12a (CRISPR/Cas12a) combined with recombinase polymerase amplification and visual detection (CRISPR/Cas12a-VD). &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;CRISPR/Cas12a-VD accurately detected target DNA at concentrations as low as 10&amp;lt;sup&amp;lt;-18&amp;lt;/sup&amp;lt; M (single molecule detection) within 30 min without cross-reactivity against other bacteria. When detecting pure cultures of VP, the consistency of results reached 100% compared with real-time PCR. The method accurately analysed pure cultures and spiked shrimp samples at concentrations as low as 10&amp;lt;sup&amp;lt;2&amp;lt;/sup&amp;lt; CFU/g. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;The novel CRISPR/Cas12a-VD method for detecting VP performed better than traditional detection methods, such as real-time PCR, and has great potential for preventing the spread of pathogens.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>518</startPage>
	      	<endPage>527</endPage>
	      	<author>
				JIANG Han Ji, TAN Rong, JIN Min, YIN Jing, GAO Zhi Xian, LI Hai Bei, SHI Dan Yang, ZHOU Shu Qing, CHEN Tian Jiao, YANG Dong, LI Jun Wen
	      	</author>
	    </item>
    	    <item>
	       	<title>Using 16S rDNA Sequencing Technology to Preliminarily Analyze Intestinal Flora in Children with &amp;lt;i&amp;lt;Mycoplasma pneumoniae&amp;lt;/i&amp;lt; Pneumonia</title>
	      	<link>//article/id/d513053f-c1ef-49d5-815e-bbfb77200bc4</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;We investigated changes in the intestinal flora of children with &amp;lt;i&amp;lt;Mycoplasma pneumoniae&amp;lt;/i&amp;lt; pneumonia (MPP). &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Between September 2019 and November 2019, stool samples from 14 children with MPP from The Fourth Hospital of Baotou city, Inner Mongolia Autonomous Region, were collected and divided into general treatment (AF) and probiotic (AFY) groups, according to the treatment of “combined &amp;lt;i&amp;lt;Bifidobacterium, Lactobacillus, Enterococcus,&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;Bacillus cereus&amp;lt;/i&amp;lt; tablets live”. High-throughput &amp;lt;i&amp;lt;16S rDNA&amp;lt;/i&amp;lt; sequencing was used to identify intestinal flora. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Intestinal flora abundance and diversity in children with MPP were decreased. Both Shannon and Simpson indices were lower in the AF group when compared with healthy controls (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.05). When compared with healthy controls, the proportion of &amp;lt;i&amp;lt;Enterorhabdus&amp;lt;/i&amp;lt; was lower in the AF group, while the proportion of &amp;lt;i&amp;lt;Lachnoclostridium&amp;lt;/i&amp;lt; was higher (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.05). The proportion of &amp;lt;i&amp;lt;Bifidobacteria&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;Akkermansia&amp;lt;/i&amp;lt; was lower in the AFY group but &amp;lt;i&amp;lt;Enterococcus&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;Lachnoclostridium&amp;lt;/i&amp;lt;, &amp;lt;i&amp;lt;Roseburia,&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;Erysipelatoclostridium&amp;lt;/i&amp;lt; proportions were higher. The proportion of &amp;lt;i&amp;lt;Escherichia coli&amp;lt;/i&amp;lt;–&amp;lt;i&amp;lt;Shigella&amp;lt;/i&amp;lt; in the AFY group after treatment was decreased (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.05). &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusions&amp;lt;/b&amp;lt; &amp;nbsp;The intestinal flora of children with MPP is disturbed, manifested as decreased abundance and diversity, and decreased &amp;lt;i&amp;lt;Bifidobacteria&amp;lt;/i&amp;lt;. Our probiotic mixture partly improved intestinal flora disorders.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>528</startPage>
	      	<endPage>537</endPage>
	      	<author>
				SHI Da Wei, WANG Dong Mei, NING Li Hua, LI Jing, DONG Yan, ZHANG Zhi Kun, DOU Hai Wei, WAN Rui Jie, JIA Chun Mei, XIN De LI
	      	</author>
	    </item>
    	    <item>
	       	<title>Effects of Hyperbaric Exposure on Hematology and Cecal Microbiota in Rats</title>
	      	<link>//article/id/8f03f679-ca89-4390-a9a1-d073d35501ca</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>538</startPage>
	      	<endPage>542</endPage>
	      	<author>
				ZHANG Chun Hong, DONG Shu Yue, LI Dan, LIU Jun, MAO Wei Chang, FANG Yi Qun, ZHANG Tie Hua
	      	</author>
	    </item>
    	    <item>
	       	<title>Evaluation of Insulin Resistance Markers and Their Relationship with &amp;lt;i&amp;lt;ADIPO Q&amp;lt;/i&amp;lt; Gene Polymorphism in Clinically Euthyroid Type 2 Diabetic Patients</title>
	      	<link>//article/id/cbb1e189-78c8-49cd-8ae1-30cafac2c6d5</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>543</startPage>
	      	<endPage>546</endPage>
	      	<author>
				Rajendran Jayanthi, Anandaraj Lokeshmaran, Ramasamy Sathiya, Abu Raghavan Srinivasan
	      	</author>
	    </item>
    	    <item>
	       	<title>Triclosan-induced Oxidative Stress Injury and Apoptosis by Regulating the PI3K/Akt/Caspase-3 Signaling Pathway in Human Renal Glomerular Endothelial Cells</title>
	      	<link>//article/id/335d1455-8a9c-4a36-8781-4687232bea51</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>547</startPage>
	      	<endPage>551</endPage>
	      	<author>
				MA Yan, CHEN Chen, WANG Jing Bo, CHENG Jia Li, SHEN Shi, CHEN Xi, HUO Jun Sheng
	      	</author>
	    </item>
    	    <item>
	       	<title>Long-Term, Low-Level Microwave Radiation Impairs Learning and Memory &amp;lt;i&amp;lt;via&amp;lt;/i&amp;lt; Synbindin: Molecular Basis and Underlying Mechanism</title>
	      	<link>//article/id/bb7bc941-0cd3-4524-b8b8-f8f259ffa3c8</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>552</startPage>
	      	<endPage>557</endPage>
	      	<author>
				YANG Zhen Zhong, WU Da Wei, DING Bai, MA Hong Bo, FEI Jin Xue, CHENG Liang, ZHAO Ya Li
	      	</author>
	    </item>
    	    <item>
	       	<title>Association of Early-Life Famine Exposure with Metabolic Dysfunction-Associated Fatty Liver Disease and Fibrosis in Adulthood</title>
	      	<link>//article/id/63ad26be-5901-43fe-a2c6-a472caad73be</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>558</startPage>
	      	<endPage>562</endPage>
	      	<author>
				WEI Ran, QI Hong Yan, LIN Lin, ZHU Yuan Yue, ZHANG Yi, ZHANG Jie, WU Xue Yan, HU Chun Yan, WANG Shuang Yuan, LIN Hong, XU Yu, XU Min, BI Yu Fang, WANG Wei Qing, LU Jie Li, NING Guang, CHEN Yu Hong
	      	</author>
	    </item>
    	    <item>
	       	<title>Now- and Fore-casting the Secular Epidemiological Trends and Seasonality of the Comeback of Scarlet Fever in China: A 16-year Time Series Analysis</title>
	      	<link>//article/id/68cb9478-9ad0-4c5a-afa7-098b42eda95e</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>563</startPage>
	      	<endPage>567</endPage>
	      	<author>
				WANG Yong Bin, LI Yan Yan, LU Hao, TAO Ying Jun, LI Yu Hong, WANG Lei, LIANG Wen Juan
	      	</author>
	    </item>
    	    <item>
	       	<title>Impact of COVID-19 Pandemic on the Mental Health of Returning Employees in Labor-Intensive Companies: A Study Based on Repeated Surveys</title>
	      	<link>//article/id/27edf460-f3d5-4bd4-886c-7defda58a997</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>6</issue>
	      	<startPage>568</startPage>
	      	<endPage>572</endPage>
	      	<author>
				LIU Yu, WANG Xiao Feng, XI Jun Yan, XIAO Qi Peng, HE Li Ping, LU Ci Yong, HE Ling
	      	</author>
	    </item>
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