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      <title>Biomedical and Environmental Sciences</title>
    <link>/</link>
    <description><![CDATA[《Biomedical and Environmental Sciences》2022年第12期]]></description>
    <year><![CDATA[2022]]></year>
    <volume><![CDATA[35]]></volume>
    <issue><![CDATA[12]]></issue>
    	    <item>
	       	<title>Cover</title>
	      	<link>//article/id/61cdbf18-fbca-44cc-bc8c-9289d35c40bb</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage></startPage>
	      	<endPage></endPage>
	      	<author>
				
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    	    <item>
	       	<title>Contents</title>
	      	<link>//article/id/963ee37c-820c-412e-97c9-03d50b8acd49</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1</startPage>
	      	<endPage>2</endPage>
	      	<author>
				
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    	    <item>
	       	<title>3- to 24-month Follow-up on COVID-19 with Pulmonary Tuberculosis Survivors after Discharge: Results from a Prospective, Multicenter Study</title>
	      	<link>//article/id/a80f777e-fa46-44b3-9e7f-b2cd5a61166e</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt;  &amp;nbsp;Coronavirus disease 2019 (COVID-19) and tuberculosis (TB) are major public health and social issues worldwide. The long-term follow-up of COVID-19 with pulmonary TB (PTB) survivors after discharge is unclear. This study aimed to comprehensively describe clinical outcomes, including sequela and recurrence at 3, 12, and 24 months after discharge, among COVID-19 with PTB survivors. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt;  &amp;nbsp;From January 22, 2020 to May 6, 2022, with a follow-up by August 26, 2022, a prospective, multicenter follow-up study was conducted on COVID-19 with PTB survivors after discharge in 13 hospitals from four provinces in China. Clinical outcomes, including sequela, recurrence of COVID-19, and PTB survivors, were collected &amp;lt;i&amp;lt;via&amp;lt;/i&amp;lt; telephone and face-to-face interviews at 3, 12, and 24 months after discharge. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt;  &amp;nbsp;Thirty-two COVID-19 with PTB survivors were included. The median age was 52 (45, 59) years, and 23 (71.9%) were men. Among them, nearly two-thirds (62.5%) of the survivors were moderate, three (9.4%) were severe, and more than half (59.4%) had at least one comorbidity (PTB excluded). The proportion of COVID-19 survivors with at least one sequela symptom decreased from 40.6% at 3 months to 15.8% at 24 months, with anxiety having a higher proportion over a follow-up. Cough and amnesia recovered at the 12-month follow-up, while anxiety, fatigue, and trouble sleeping remained after 24 months. Additionally, one (3.1%) case presented two recurrences of PTB and no re-positive COVID-19 during the follow-up period. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp;  &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt;  &amp;nbsp;The proportion of long symptoms in COVID-19 with PTB survivors decreased over time, while nearly one in six still experience persistent symptoms with a higher proportion of anxiety. The recurrence of PTB and the psychological support of COVID-19 with PTB after discharge require more attention.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1091</startPage>
	      	<endPage>1099</endPage>
	      	<author>
				WANG Jing Ya, ZONG Xing Yu, WU Gui Hui, QI Yuan Lin, LI Hui Zhen, JI Xin Yu, TONG Lin, ZHANG Lei, YANG Ming Bo, YANG Pu Ye, LI Ji Ke, XIAO Fu Rong, ZHANG Lin Song, HU Yun Hong, LIU Hong De, XU Shou Fang, SUN Sheng, WU Wei, MAO Ya, LI Min Qing, HOU Hao Hua, GONG Zhao Yuan, GUO Yang, JIAO Li Wen, QIN Jin, WANG Ding Yi, WANG Fang, GUAN Li, LIN Gang, MA Yan, WANG Yan Ping, SHI Nan Nan
	      	</author>
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    	    <item>
	       	<title>Symptomatic and Asymptomatic SARS-CoV-2 Infection and Follow-up of Neutralizing Antibody Levels</title>
	      	<link>//article/id/3071e628-8c6e-4df2-bc3c-48d3be212f61</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;To investigate neutralizing antibody levels in symptomatic and asymptomatic patients with coronavirus disease 2019 (COVID-19) at 6 and 10 months after disease onset. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Blood samples were collected at three different time points from 27 asymptomatic individuals and 69 symptomatic patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Virus-neutralizing antibody titers against SARS-CoV-2 in both groups were measured and statistically analyzed. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;The symptomatic and asymptomatic groups had higher neutralizing antibodies at 3 months and 1–2 months post polymerase chain reaction confirmation, respectively. However, neutralizing antibodies in both groups dropped significantly to lower levels at 6 months post-PCR confirmation. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;Continued monitoring of symptomatic and asymptomatic individuals with COVID-19 is key to controlling the infection.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1100</startPage>
	      	<endPage>1105</endPage>
	      	<author>
				CUI Shu Juan, ZHANG Yi, GAO Wen Jing, WANG Xiao Li, YANG Peng, WANG Quan Yi, PANG Xing Huo, ZENG Xiao Peng, LI Li Ming
	      	</author>
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    	    <item>
	       	<title>Genetic Characteristics of Lipooligosaccharide and Capsular Polysaccharide of &amp;lt;i&amp;lt;Campylobacter jejuni&amp;lt;/i&amp;lt; from Different Sources in China</title>
	      	<link>//article/id/f84cd2c1-5a97-4578-9e1e-7e5ddd9fe394</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;To determine the distribution of two important virulence factors [lipooligosaccharide (LOS) and capsular polysaccharide (CPS)] in &amp;lt;i&amp;lt;Campylobacter jejuni&amp;lt;/i&amp;lt; (&amp;lt;i&amp;lt;C. jejuni&amp;lt;/i&amp;lt;) isolated from different sources in China and to develop a rapid screening method for Guillain–Barré syndrome (GBS)-associated strains. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;Whole-genome sequencing was carried out for 494 &amp;lt;i&amp;lt;C. jejuni&amp;lt;/i&amp;lt; strains. The OrthoMCL software was used to define the LOS/CPS gene clusters. CPS genotyping was performed with serotype-specific sequence alignment using the BLAST software. Real-time Polymerase chain reaction (PCR) was developed with the unique sequences of specific CPS types. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Nine novel and 29 previously confirmed LOS classes were identified. LOS classes A, B, and C were the most common (48.2%, 238/494) among the 494 strains. Twenty-six capsular types were identified in 448 strains. HS2, HS4c, HS5/31, HS19, and HS8/17 were the most frequent CPS genotypes (58.7%, 263/448). Strains of 17 CPS genotypes (strain number &amp;gt; 5) had one or two prevalent LOS classes (&amp;lt;i&amp;lt;P&amp;lt;/i&amp;lt; &amp;lt; 0.05). Multiplex real-time PCR for rapid identification of HS2, HS19, and HS41 was developed and validated with strains of known serotypes. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;Our results describe the genetic characteristics of the important virulence factors in &amp;lt;i&amp;lt;C. jejuni&amp;lt;/i&amp;lt; strains in China. The multiplex real-time PCR developed in this study will facilitate enhanced surveillance of GBS-associated strains in China.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1106</startPage>
	      	<endPage>1114</endPage>
	      	<author>
				WANG Jia Qi, CHEN Xiao Li, ZHOU Gui Lan, WANG Hai Rui, GU Yi Xin, ZHANG Jian Zhong, SHAO Zhu Jun, ZHANG Mao Jun
	      	</author>
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    	    <item>
	       	<title>Tetrahedral DNA Nanostructure-modified Gold Nanorod-based Anticancer Nanomaterials for Combined Photothermal Therapy and Chemotherapy</title>
	      	<link>//article/id/4df4d7c4-a127-41bd-b02e-a2b36757943f</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp;To develop an effective treatment strategy to simultaneously avoid fatal adverse effects in the treatment of oral cancer, combination therapy has been explored because of its multiple functions. This work aims to develop a novel type of gold-nanorod-based nanomaterials decorated with tetrahedral DNA nanostructures (TDN) carrying antitumor drugs, namely, GNR@TDN-DOX nanocomposites. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp;In the designed structure, TDN, with a three-dimensional geometry composed of DNA strands, can provide GC base pairs for binding with the anticancer drug doxorubicin (DOX). The photothermal heating properties, biocompatibility properties, and antitumor performance of obtained GNR@TDN-DOX nanocomposites were investigated to assess their application potential in tumor treatment. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp;Systematic studies have shown that the obtained GNR@TDN-DOX nanocomposites have high photothermal conversion under the illumination of an 808-nm infrared laser, leading to effective antitumor applications. In addition, the cell viability study shows that GNR@TDN-DOX nanocomposites have good biocompatibility. &amp;lt;i&amp;lt;In vitro&amp;lt;/i&amp;lt; studies based on A375 cells show that the GNR@TDN-DOX nanocomposites can effectively eliminate cancer cells because of the combination of photothermal therapy induced by GNRS and chemotherapy induced by TDN-carrying DOX. The result shows that the obtained GNR@TDN-DOX nanocomposites have efficient cellular uptake and lysosome escape ability, together with their nuclear uptake behavior, which results in a significant antitumor effect. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp;This work has demonstrated a potential nanoplatform for anticancer applications.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1115</startPage>
	      	<endPage>1125</endPage>
	      	<author>
				WU Hao, XU Jiang Shan, LI Yan Hong, WU Xing Han, HU Wei, LIU Meng Die, SUN Qiang, GUO Bin
	      	</author>
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    	    <item>
	       	<title>Influence of Built Environment in Hygienic City in China on Self-rated Health of Residents</title>
	      	<link>//article/id/8e19e147-2e31-461c-b5ed-291f71d7d71b</link>
	     	<description><![CDATA[&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Objective&amp;lt;/b&amp;lt; &amp;nbsp; To assess the subjective perception of residents on the built environment in hygienic cities and its relation to the self-rated health (SRH) status of residents, providing a basis for a better promotion on construction of health-supportive environments. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Methods&amp;lt;/b&amp;lt; &amp;nbsp; The online survey was adopted with the respondents recruited from residents living in Chaoyang District of Beijing in January 2021. With SRH level as the dependent variable, two-category logistic regression analysis was conducted to analyze the impact of the built environment in hygienic cities on the SRH status of residents. &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Results&amp;lt;/b&amp;lt; &amp;nbsp; A total of 1,357 respondents were enrolled in this study. After controlling confounding factors, four aspects in the built environment in hygienic cities were detected remarkable influences on the SRH level of residents,  including enough green space in the living area [odds ratio (&amp;lt;i&amp;lt;OR&amp;lt;/i&amp;lt;) = 1.395, 95% confidence interval (95% &amp;lt;i&amp;lt;CI&amp;lt;/i&amp;lt;): 1.055–1.845], clean and hygienic living environment (&amp;lt;i&amp;lt;OR&amp;lt;/i&amp;lt; = 1.472, 95% &amp;lt;i&amp;lt;CI&amp;lt;/i&amp;lt;: 1.107–1.956), residents&apos; confidence in drinking water safety in the living area (&amp;lt;i&amp;lt;OR&amp;lt;/i&amp;lt; = 1.856, 95% &amp;lt;i&amp;lt;CI&amp;lt;/i&amp;lt;: 1.354–2.544) and residents&apos; confidence in food safety in the living area (&amp;lt;i&amp;lt;OR&amp;lt;/i&amp;lt; = 1.405, 95% &amp;lt;i&amp;lt;CI&amp;lt;/i&amp;lt;: 1.027–1.921). &amp;lt;/sec&amp;lt;&amp;lt;sec&amp;lt;&amp;nbsp; &amp;lt;b&amp;lt;Conclusion&amp;lt;/b&amp;lt; &amp;nbsp; Regarding city construction, the government should focus more on the subjective perception of residents on built environments to build a supportive environment benefiting the health of residents.&amp;lt;/sec&amp;lt;]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1126</startPage>
	      	<endPage>1132</endPage>
	      	<author>
				ZHENG Wen Jing, QI Xiao, YAO Hong Yan, LIU Jian Jun, YU Shi Cheng, ZHANG Tao
	      	</author>
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    	    <item>
	       	<title>The Epidemiology, Diagnosis and Prognosis of Long-COVID</title>
	      	<link>//article/id/f852db03-57df-4df7-8182-d2128942371b</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1133</startPage>
	      	<endPage>1139</endPage>
	      	<author>
				DU Ying, ZHANG Jie, WU Li Juan, ZHANG Qun, WANG You Xin
	      	</author>
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	       	<title>Quality of Life in Terms of Mental Stress and Physical Activities among Community Elder Residents in Shenzhen in the Post-COVID-19 Period</title>
	      	<link>//article/id/36fbe662-cf61-4868-be63-03d3b4a1c82b</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1140</startPage>
	      	<endPage>1144</endPage>
	      	<author>
				ZHANG Xiao Ying, DENG Shu, LU Sen, ZHANG Xu Chang, NIU Tian Shui, LYU Zhong Wei, YANG Jian She
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    	    <item>
	       	<title>Comparison of Obesity Prevalence among Middle and High School Graduates before and after the COVID-19 Lockdown</title>
	      	<link>//article/id/6bce755b-8511-47f9-be38-dc3787f931c1</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1145</startPage>
	      	<endPage>1149</endPage>
	      	<author>
				LU Yan Hui, DOU Zi Yan, ZHAO Kai Ping, YAN Dong, CHEN Shuo, CHENG Jun Xia, Glen M. BLAKE, Karen HIND, ZHANG Jing Bo, CHENG Xiao Guang, WU Jing
	      	</author>
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    	    <item>
	       	<title>Incidence and Risk Factors of Progression from Prehypertension or Hypertension to Diabetes among Older Adults of Northeastern China: A Prospective Cohort Study</title>
	      	<link>//article/id/4491511a-2117-447f-a144-faeab535ee98</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1150</startPage>
	      	<endPage>1155</endPage>
	      	<author>
				LIANG Zhuo Shuai, SHI Ji Kang, TIAN Yu Yang, HU Xin Meng, REN Ya Xuan, LIU Sai Nan, WANG Yu Jian, CHENG Yi, LIU Ya Wen
	      	</author>
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	       	<title>Identification and Prediction of Diabetic Macular Edema on Two-dimensional Fundus Images with Raphe Assessment and Deep Convolution Neural Networks</title>
	      	<link>//article/id/3d509766-be5f-4c51-9c0d-d89299527263</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1156</startPage>
	      	<endPage>1160</endPage>
	      	<author>
				S. Karkuzhali
	      	</author>
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    	    <item>
	       	<title>Duplex Detection of &amp;lt;i&amp;lt;Vibrio&amp;lt;/i&amp;lt; &amp;lt;i&amp;lt;Cholerae&amp;lt;/i&amp;lt; and &amp;lt;i&amp;lt;Vibrio&amp;lt;/i&amp;lt; &amp;lt;i&amp;lt;Parahaemolyticus&amp;lt;/i&amp;lt; by Real-time Recombinase Polymerase Amplification</title>
	      	<link>//article/id/01554ee2-f652-49f0-aad7-9117a311af43</link>
	     	<description><![CDATA[]]></description>
	      	<volume>35</volume>
	      	<issue>12</issue>
	      	<startPage>1161</startPage>
	      	<endPage>1165</endPage>
	      	<author>
				LIAO Lei, SHI Wen, MA Chao, TANG Wen Lian, QIAN Qi Lan, WANG Yu, LI Jiao Jiao, SHEN Jin Yang, JI Jing, MA Jin Ming, GAO Song
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